Author: Daniel J Butler; Christopher Mozsary; Cem Meydan; David C Danko; Jonathan Foox; Joel Rosiene; Alon Shaiber; Ebrahim Afshinnekoo; Matthew MacKay; Fritz J Sedlazeck; Nikolay A Ivanov; Maria A Sierra; Diana Pohle; Michael Zeitz; Vijendra Ramlall; Undina Gisladottir; Craig D Westover; Krista Ryon; Benjamin Young; Chandrima Bhattacharya; Phyllis Ruggiero; Bradley W Langhorst; Nathan A Tanner; Justyn Gawrys; Dmitry Meleshko; Dong Xu; Jenny Xiang; Angelika Iftner; Daniela Bezdan; John Sipley; Lin Cong; Arryn Craney; Priya Velu; Ari Melnick; Iman A Hajirasouliha; Thomas Iftner; Mirella Salvatore; Massimo Loda; Lars F Westblade; Shawn Levy; Melissa Cushing; Nicholas P Tatonetti; Marcin Imielinski; Hanna Rennert; Christopher Mason
Title: Host, Viral, and Environmental Transcriptome Profiles of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Document date: 2020_4_20
ID: kyoa5gsf_44
Snippet: Clinical samples were extracted as described above and then tested with qRT-PCR using primers for the E (envelope) gene, which detects all members of the lineage B of the beta-CoVs, including all SARS, SARS-like, and SARS-related viruses, and a second primer set for the S (spike) gene, which specifically detects the SARS-CoV-2 virus. The reaction also contains an internal control that served as an extraction control and a control for PCR inhibiti.....
Document: Clinical samples were extracted as described above and then tested with qRT-PCR using primers for the E (envelope) gene, which detects all members of the lineage B of the beta-CoVs, including all SARS, SARS-like, and SARS-related viruses, and a second primer set for the S (spike) gene, which specifically detects the SARS-CoV-2 virus. The reaction also contains an internal control that served as an extraction control and a control for PCR inhibition.
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