Author: Ben Longdon; Jonathan P Day; Joel M Alves; Sophia CL Smith; Thomas M Houslay; John E McGonigle; Lucia Tagliaferri; Francis M Jiggins
Title: Host shifts result in parallel genetic changes when viruses evolve in closely related species Document date: 2017_11_28
ID: ithoxu6k_33
Snippet: B6A, was selected for amplification and grown in cell culture as described above. Media containing 203 DCV was removed and centrifuged at 3000 x g for 5 minutes at 4°C to pellet any remaining cell 204 . CC-BY-NC 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/226175 doi: bioRxiv preprint 8 debris, before being aliquoted and.....
Document: B6A, was selected for amplification and grown in cell culture as described above. Media containing 203 DCV was removed and centrifuged at 3000 x g for 5 minutes at 4°C to pellet any remaining cell 204 . CC-BY-NC 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/226175 doi: bioRxiv preprint 8 debris, before being aliquoted and stored at -80°C. The Tissue Culture Infective Dose 50 (TCID 50 ) of 205 the DCV was 6.32 x 10 9 infectious particles per ml using the .
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