Selected article for: "analytical sensitivity and detection limit analytical sensitivity"
Author: Daniel J Butler; Christopher Mozsary; Cem Meydan; David C Danko; Jonathan Foox; Joel Rosiene; Alon Shaiber; Ebrahim Afshinnekoo; Matthew MacKay; Fritz J Sedlazeck; Nikolay A Ivanov; Maria A Sierra; Diana Pohle; Michael Zeitz; Vijendra Ramlall; Undina Gisladottir; Craig D Westover; Krista Ryon; Benjamin Young; Chandrima Bhattacharya; Phyllis Ruggiero; Bradley W Langhorst; Nathan A Tanner; Justyn Gawrys; Dmitry Meleshko; Dong Xu; Jenny Xiang; Angelika Iftner; Daniela Bezdan; John Sipley; Lin Cong; Arryn Craney; Priya Velu; Ari Melnick; Iman A Hajirasouliha; Thomas Iftner; Mirella Salvatore; Massimo Loda; Lars F Westblade; Shawn Levy; Melissa Cushing; Nicholas P Tatonetti; Marcin Imielinski; Hanna Rennert; Christopher Mason
Title: Host, Viral, and Environmental Transcriptome Profiles of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
  • Document date: 2020_4_20
    • ID: kyoa5gsf_4
    Snippet: To validate the assay, we first evaluated two synthetic RNAs (see Methods) whose sequences matched the viral sequences of patients from Wuhan, China and Melbourne, Australia (Supp. Fig. 1 ). The first control (MT007544.1) was used to test the analytical sensitivity via the limit of detection (LoD), titrated from 1 million molecules of virus (10 6 ) down to a single copy, using serial log-10 dilutions. The reaction output was measured at 0-, 20-, .....
    Document: To validate the assay, we first evaluated two synthetic RNAs (see Methods) whose sequences matched the viral sequences of patients from Wuhan, China and Melbourne, Australia (Supp. Fig. 1 ). The first control (MT007544.1) was used to test the analytical sensitivity via the limit of detection (LoD), titrated from 1 million molecules of virus (10 6 ) down to a single copy, using serial log-10 dilutions. The reaction output was measured at 0-, 20-, and 30-minute intervals (Figure 1c, Supp. Figure 2a) before the samples were heated to 95 o C for inactivation. The LoD was found to be between 10-100 viral total copies, and this was then replicated to show a similar LoD of 10-100 viral copies on the second synthetic control from the patient from Wuhan (Supp. Figure 2b) .

    Search related documents:
    Co phrase search for related documents
    • analytical sensitivity and LoD detection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • analytical sensitivity and LoD detection limit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • analytical sensitivity and LoD detection limit analytical sensitivity test: 1
    • analytical sensitivity and serial dilution: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • analytical sensitivity and similar lod: 1
    • analytical sensitivity and single copy: 1
    • analytical sensitivity and synthetic control: 1, 2
    • analytical sensitivity and synthetic rna: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • analytical sensitivity and total copy: 1
    • analytical sensitivity and viral copy: 1
    • analytical sensitivity and viral sequence: 1, 2
    • analytical sensitivity test and LoD detection: 1, 2, 3, 4
    • analytical sensitivity test and LoD detection limit: 1, 2, 3, 4
    • analytical sensitivity test and LoD detection limit analytical sensitivity test: 1
    • analytical sensitivity test and synthetic rna: 1
    • assay validate and synthetic rna: 1
    • LoD detection and synthetic rna: 1, 2, 3, 4, 5, 6
    • LoD detection and viral copy: 1, 2, 3
    • LoD detection and viral sequence: 1, 2