Selected article for: "negative control and positive control"

Author: Daniel J Butler; Christopher Mozsary; Cem Meydan; David C Danko; Jonathan Foox; Joel Rosiene; Alon Shaiber; Ebrahim Afshinnekoo; Matthew MacKay; Fritz J Sedlazeck; Nikolay A Ivanov; Maria A Sierra; Diana Pohle; Michael Zeitz; Vijendra Ramlall; Undina Gisladottir; Craig D Westover; Krista Ryon; Benjamin Young; Chandrima Bhattacharya; Phyllis Ruggiero; Bradley W Langhorst; Nathan A Tanner; Justyn Gawrys; Dmitry Meleshko; Dong Xu; Jenny Xiang; Angelika Iftner; Daniela Bezdan; John Sipley; Lin Cong; Arryn Craney; Priya Velu; Ari Melnick; Iman A Hajirasouliha; Thomas Iftner; Mirella Salvatore; Massimo Loda; Lars F Westblade; Shawn Levy; Melissa Cushing; Nicholas P Tatonetti; Marcin Imielinski; Hanna Rennert; Christopher Mason
Title: Host, Viral, and Environmental Transcriptome Profiles of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
  • Document date: 2020_4_20
  • ID: kyoa5gsf_52
    Snippet: Completed reactions were analyzed with the Promega GloMax Explorer (Promega GM3500) fluorometer using the QuantiFluor ONE dsDNA system (Promega E4871). This system recorded light intensity from each well using an emission filter of 500-550nm, an excitation filter set at blue 475 nm, and a high sensitivity setting on the Glomax software. Values were then tabulated and compared with controls (positive and negative). The intensity threshold of 2.5x .....
    Document: Completed reactions were analyzed with the Promega GloMax Explorer (Promega GM3500) fluorometer using the QuantiFluor ONE dsDNA system (Promega E4871). This system recorded light intensity from each well using an emission filter of 500-550nm, an excitation filter set at blue 475 nm, and a high sensitivity setting on the Glomax software. Values were then tabulated and compared with controls (positive and negative). The intensity threshold of 2.5x negative control was used as the threshold for positive detection.

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