Author: Aleksandra Milewska; Ying Chi; Artur Szczepanski; Emilia Barreto-Duran; Kevin Liu; Dan Liu; Xiling Guo; Yiyue Ge; Jingxin Li; Lunbiao Cui; Marek Ochman; Maciej Urlik; Sylwia Rodziewicz-Motowidlo; Fengcai Zhu; Krzysztof Szczubialka; Maria Nowakowska; Krzysztof Pyrc
Title: HTCC as a highly effective polymeric inhibitor of SARS-CoV-2 and MERS-CoV Document date: 2020_3_31
ID: 86stuueg_13
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.29.014183 doi: bioRxiv preprint presence of tested polymer or control medium. Following a 2 h incubation at 37°C, unbound 312 virions were removed by washing with 100 μl of 1 × PBS and fresh medium containing 313 dissolved respective polymer was added to each well. Samples of cell culture supernatant were 314 collected at day 3 p.i. an.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.29.014183 doi: bioRxiv preprint presence of tested polymer or control medium. Following a 2 h incubation at 37°C, unbound 312 virions were removed by washing with 100 μl of 1 × PBS and fresh medium containing 313 dissolved respective polymer was added to each well. Samples of cell culture supernatant were 314 collected at day 3 p.i. and analyzed using RT-qPCR. 315 For the ex vivo study, fully differentiated human airway epithelium (HAE) cultures were 316 exposed to the tested polymer or control PBS for 30 min at 37°C, following inoculation with The copyright holder for this preprint (which was not peer-reviewed) is the . (Scientific Volume Imaging B.V., The Netherlands) and processed using ImageJ 1.47v 358 author/funder. All rights reserved. No reuse allowed without permission.
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