Author: Amir Saberi; Anastasia A. Gulyaeva; John L. Brubacher; Phillip A. Newmark; Alexander E. Gorbalenya
Title: A planarian nidovirus expands the limits of RNA genome size Document date: 2018_4_11
ID: gwgflckq_28
Snippet: In situ hybridization. Colorimetric and fluorescent in situ hybridizations were done following published methods (61) . Digoxigenin (DIG)-labelled PSCNV probes were generated by antisense transcription of the planarian EST clone PL06016B2F06 (GenBank DN313906.1) (26). Following color development, all samples were cleared in 80% (v/v) glycerol and imaged on a Leica M205A microscope (colorimetric) or a Carl Zeiss LSM710 confocal microscope (fluores.....
Document: In situ hybridization. Colorimetric and fluorescent in situ hybridizations were done following published methods (61) . Digoxigenin (DIG)-labelled PSCNV probes were generated by antisense transcription of the planarian EST clone PL06016B2F06 (GenBank DN313906.1) (26). Following color development, all samples were cleared in 80% (v/v) glycerol and imaged on a Leica M205A microscope (colorimetric) or a Carl Zeiss LSM710 confocal microscope (fluorescent).
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