Author: Longlong Si; Haiqing Bai; Melissa Rodas; Wuji Cao; Crystal Yur Oh; Amanda Jiang; Atiq Nurani; Danni Y Zhu; Girija Goyal; Sarah Gilpin; Rachelle Prantil-Baun; Donald E. Ingber
Title: Human organs-on-chips as tools for repurposing approved drugs as potential influenza and COVID19 therapeutics in viral pandemics Document date: 2020_4_14
ID: mrgw2mnx_41
Snippet: To measure tissue barrier permeability, 50 µl cell medium containing Cascade blue (607 Da) (50 µg/mL; Invitrogen) was added to bottom channel and 50 µl cell medium was added to top channel. The fluorescence intensity of medium of top and bottom channels was measured 2 h later in three different human Airway chips. The apparent permeability was calculated using the formula: Papp = J/(A × author/funder. All rights reserved. No reuse allowed wit.....
Document: To measure tissue barrier permeability, 50 µl cell medium containing Cascade blue (607 Da) (50 µg/mL; Invitrogen) was added to bottom channel and 50 µl cell medium was added to top channel. The fluorescence intensity of medium of top and bottom channels was measured 2 h later in three different human Airway chips. The apparent permeability was calculated using the formula: Papp = J/(A × author/funder. All rights reserved. No reuse allowed without permission.
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