Author: Hema Kothari; Corey M. Williams; Chantel McSkimming; Mythili Vigneshwar; Eli R. Zunder; Coleen A. McNamara
Title: Identification of Human Immune Cell Subtypes Most Vulnerable to IL-1ß-induced Inflammatory Signaling Using Mass Cytometry Document date: 2020_4_19
ID: 5rhn7gom_8
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.19.047274 doi: bioRxiv preprint subsequent experiments. To map IL-1β-induced signaling responses across major immune cell types, Spanning-tree Progression Analysis of Density-normalized Events (SPADE) analysis was applied on all vehicle and IL-1β-stimulated samples. SPADE analysis identified 9 major immune cell populations including, CD.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.19.047274 doi: bioRxiv preprint subsequent experiments. To map IL-1β-induced signaling responses across major immune cell types, Spanning-tree Progression Analysis of Density-normalized Events (SPADE) analysis was applied on all vehicle and IL-1β-stimulated samples. SPADE analysis identified 9 major immune cell populations including, CD4 + , CD8 + , and CD4 -CD8 low T cells, CD19 + B cells, HLA-DR + CD14 + and HLA-DR + CD16 + monocytes, HLA-DR + CD11c + (mDCs) and HLA-DR + CD123 + plasmacytoid DCs (pDCs), and HLA-DR -CD56 + CD16 + NK cells (Fig. 1c,d) . CD4 T cells are the most abundant cell type within PBMCs (Fig. 1d) .
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