Author: Chenyu Li; David N. Debruyne; Julia Spencer; Vidushi Kapoor; Lily Y. Liu; Bo Zhou; Lucie Lee; Rounak Feigelman; Grayson Burdon; Jeffrey Liu; Alejandra Oliva; Adam Borcherding; Hongdong Tan; Alexander E. Urban; Guoying Liu; Zhitong Liu
Title: High sensitivity detection of coronavirus SARS-CoV-2 using multiplex PCR and a multiplex-PCR-based metagenomic method Document date: 2020_3_14
ID: 0hxan9rw_11
Snippet: The workflow was designed so that the multiplex PCR products are further amplified in a secondary PCR, while sample indexes and NGS sequencing primers are added (Fig. 1D) . The PCR products were first analyzed by electrophoresis to identify potential positives. Since dozens of amplicons could be amplified from a single copy of SARS-CoV-2, electrophoresis peaks with defined peak sizes were expected. Multiplex PCR amplifies SARS-CoV-2, as well as o.....
Document: The workflow was designed so that the multiplex PCR products are further amplified in a secondary PCR, while sample indexes and NGS sequencing primers are added (Fig. 1D) . The PCR products were first analyzed by electrophoresis to identify potential positives. Since dozens of amplicons could be amplified from a single copy of SARS-CoV-2, electrophoresis peaks with defined peak sizes were expected. Multiplex PCR amplifies SARS-CoV-2, as well as other coronaviruses due to their high sequence similarities. In that context, electrophoresis analysis provides a fast and sensitive indication of infection from that family of viruses. In addition, the generated library allows for further investigation through NGS sequencing to provide definitive identification of the specific virus family member.
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