Correlation of mRNA delivery timing and protein expression in lipid-based transfection

A. Reiser; D. Woschée; N. Mehrotra; R. Krzyszton; H. H. Strey; J. O. Rädler

The Lipofectamine data show that both onset time and efficiency depend on serum concentration. However, we will demonstrate that this relation depends on the type of mRNA carrier and is not intrinsically linked. Comparing single-cell data from Lipofectamine transfection with data obtained after transfecting with LNPs made from the ionizable lipid Dlin-MC3-DMA (Materials and Methods) we observe the opposite behavior (see Figure 5 ). Using Lipofectamine in the presence of FBS, the distributions are shifted to longer onset times and lower expression level (see first plot of Figure 5 ). For cells transfected with LNPs the opposite trend is the case (see second plot of Figure 5 ), the onset times shortens and the expression level increases. Furthermore, we observe a more homogenous behavior for cells treated with LNPs compared to Lipofectamine treated cells, which can be seen in the less scattered LNP data.