Author: A. Reiser; D. Woschée; N. Mehrotra; R. Krzyszton; H. H. Strey; J. O. Rädler
Title: Correlation of mRNA delivery timing and protein expression in lipid-based transfection Document date: 2019_4_13
ID: 9h6ctbyx_23
Snippet: Using microarrays, we measured single-cell eGFP expression time courses and obtained single-cell expression rate and onset time distribution functions for mRNA transfection. The onset times display the total delivery time, which includes multiple intermediate steps consisting of mRNA nanocarrier addition to the single-cell microarray, particle adsorption on the cell surface, particle uptake, endosomal release, and the measured protein expression .....
Document: Using microarrays, we measured single-cell eGFP expression time courses and obtained single-cell expression rate and onset time distribution functions for mRNA transfection. The onset times display the total delivery time, which includes multiple intermediate steps consisting of mRNA nanocarrier addition to the single-cell microarray, particle adsorption on the cell surface, particle uptake, endosomal release, and the measured protein expression onset. To avoid uncontrolled long-term adsorption of complexes we used a perfusion system to apply a defined incubation period of 1 h (see Supplementary Figure 1B ) during the timelapse measurement. This enables us to cover the full dynamic range of measurable onset author/funder. All rights reserved. No reuse allowed without permission.
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