Author: Rashmi Mohanty; Xinquan Liu; Jae You Kim; Xiujuan Peng; Sahil Bhandari; Jasmim Leal; Dhivya Arasappan; Dennis C. Wylie; Tony Dong; Debadyuti Ghosh
Title: Identification of peptide coatings that enhance diffusive transport of nanoparticles through the tumor microenvironment Document date: 2019_6_4
ID: e2uzk4u1_80
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/659524 doi: bioRxiv preprint Five peptide sequences were selected from the NGS analysis and sequence alignment to be validated for transport through tumor ECM. In addition, a negative control clone was chosen from the original library, which was not enriched with increased rounds of selection; instead, its frequency decreased with .....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/659524 doi: bioRxiv preprint Five peptide sequences were selected from the NGS analysis and sequence alignment to be validated for transport through tumor ECM. In addition, a negative control clone was chosen from the original library, which was not enriched with increased rounds of selection; instead, its frequency decreased with successive rounds. The peptides were each genetically engineered to be displayed on T7 based on the manufacturer's protocol (MilliporeSigma). Briefly, the reverse complement oligonucleotides encoding each peptide sequences and the biotinylated forward oligo
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