Identification of peptide coatings that enhance diffusive transport of nanoparticles through the tumor microenvironment

Rashmi Mohanty; Xinquan Liu; Jae You Kim; Xiujuan Peng; Sahil Bhandari; Jasmim Leal; Dhivya Arasappan; Dennis C. Wylie; Tony Dong; Debadyuti Ghosh

Briefly, the spatial fluorescence intensity was recorded from the average intensity over each 5pixel penetration length of the channel (up to 1.1 mm from the input reservoir) and the entire width of the channel for all the time-lapse images. The background intensity for each channel was subtracted from the spatiotemporal intensity profile and was then normalized based on the maximum fluorescence intensity. The intensity decay was plotted along with the penetration length after 6 hours of diffusion. The average diffusion coefficient of the clones was calculated from the non-linear curve fitting of each experimental normalized intensity decay values with the onedimensional analytical Fick's second law of diffusion (1). The diffusion coefficient was assumed to be independent of the concentration of the clone and the penetration depth.