Selected article for: "filtration column and room temperature"
Author: Renata C Fleith; Harriet V Mears; Edward Emmott; Stephen C Graham; Daniel S Mansur; Trevor R Sweeney
Title: IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA
  • Document date: 2018_2_8
    • ID: j97gul0w_15
    Snippet: Proteins/complexes were injected (100 L at the concentration described in IFIT complex assembly, above) onto an analytical Superdex 200 Increase 10/300 gel filtration column. MALS analysis was performed at room temperature, by inline measurement of static light scattering (DAWN 8+, Wyatt Technology), differential refractive index (Optilab T-rEX, Wyatt Technology), and 280 nm absorbance (Agilent 1260 UV, Agilent Technologies) following SEC at a.....
    Document: Proteins/complexes were injected (100 L at the concentration described in IFIT complex assembly, above) onto an analytical Superdex 200 Increase 10/300 gel filtration column. MALS analysis was performed at room temperature, by inline measurement of static light scattering (DAWN 8+, Wyatt Technology), differential refractive index (Optilab T-rEX, Wyatt Technology), and 280 nm absorbance (Agilent 1260 UV, Agilent Technologies) following SEC at a flow rate of 0.4 mL/min. Molecular masses were calculated using the ASTRA6 software package (Wyatt Technology).

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