Author: Amir Saberi; Anastasia A. Gulyaeva; John L. Brubacher; Phillip A. Newmark; Alexander E. Gorbalenya
Title: A planarian nidovirus expands the limits of RNA genome size Document date: 2018_4_11
ID: gwgflckq_27
Snippet: Freshly prepared RNA from mature sexual planarians was used for cDNA synthesis (iScript, Bio-Rad) or 5'-RACE (RLM-RACE, Ambion) according to manufacturer instructions. Large overlapping amplicons across the PSCNV genome (primers in Table S2 ) were amplified by standard Phusion® High-Fidelity DNA polymerase reaction with 65ºC primer annealing temperature and 10 min extension steps......
Document: Freshly prepared RNA from mature sexual planarians was used for cDNA synthesis (iScript, Bio-Rad) or 5'-RACE (RLM-RACE, Ambion) according to manufacturer instructions. Large overlapping amplicons across the PSCNV genome (primers in Table S2 ) were amplified by standard Phusion® High-Fidelity DNA polymerase reaction with 65ºC primer annealing temperature and 10 min extension steps.
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