A comprehensive library of fluorescent constructs of SARS‐CoV‐2 proteins and their initial characterization in different cell types

Miserey‐Lenkei, Stéphanie; Trajkovic, Katarina; D'Ambrosio, Juan Martín; Patel, Amanda J; Čopič, Alenka; Mathur, Pallavi; Schauer, Kristine; Goud, Bruno; Albanèse, Véronique; Gautier, Romain; Subra, Melody; Kovacs, David; Barelli, Hélène; Antonny, Bruno

Comprehensive libraries of plasmids for SARS‐CoV‐2 proteins with various tags (e.g. Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein‐protein interactions between the SARS‐CoV‐2 virus and host proteins. To facilitate further cellular investigations, notably by imaging techniques, we present here a large library of SARS CoV‐2 protein constructs fused with green and red fluorescent proteins and their initial characterization in various human cell lines including lung epithelial cell models (A549, BEAS‐2B), as well as in budding yeast. The localization of a few SARS‐CoV‐2 proteins matches their proposed interactions with host proteins. These include the localization of Nsp13 to the centrosome, Orf3a to late endosomes, and Orf9b to mitochondria. This article is protected by copyright. All rights reserved