Author: Mahesh K R Kalikiri; Mohammad Rubayet Hasan; Faheem Mirza; Thabisile Xaba; Patrick Tang; Stephan Lorenz
Title: High-throughput extraction of SARS-CoV-2 RNA from nasopharyngeal swabs using solid-phase reverse immobilization beads Document date: 2020_4_11
ID: bx2xspbe_10
Snippet: When adding between 0 and 1 µg carrier RNA in 0.25 µg increments directly into the one-step RT-qPCR reaction assay detecting a SARS-CoV-2 positive control, we observed a decrease in Cq directly correlated to the amount of carrier RNA (Pearson r = 0.985, Figure 2 ) present in the reaction. For all subsequent experiments we added 0.5 µg of carrier RNA per extraction sample, mixed into the RLT buffer......
Document: When adding between 0 and 1 µg carrier RNA in 0.25 µg increments directly into the one-step RT-qPCR reaction assay detecting a SARS-CoV-2 positive control, we observed a decrease in Cq directly correlated to the amount of carrier RNA (Pearson r = 0.985, Figure 2 ) present in the reaction. For all subsequent experiments we added 0.5 µg of carrier RNA per extraction sample, mixed into the RLT buffer.
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