Author: Martin Mikl; Yitzhak Pilpel; Eran Segal
Title: High-throughput interrogation of programmed ribosomal frameshifting in human cells Document date: 2018_11_14
ID: 5zjnzsik_2
Snippet: Fameshifting is generally believed to happen at defined positions consisting of a slippery sequence 51 and a downstream roadblock, most commonly a stable secondary RNA structure like a pseudoknot 52 or an extensive stem-loop structure (Caliskan et al., 2015; Dinman, 2012) . Most presently known -1 53 slippery sites follow the pattern X XXY YYZ, with the shift happening from codons XXY and YYZ to 54 XXX and YYY. Some known slippery sites in -1 PRF.....
Document: Fameshifting is generally believed to happen at defined positions consisting of a slippery sequence 51 and a downstream roadblock, most commonly a stable secondary RNA structure like a pseudoknot 52 or an extensive stem-loop structure (Caliskan et al., 2015; Dinman, 2012) . Most presently known -1 53 slippery sites follow the pattern X XXY YYZ, with the shift happening from codons XXY and YYZ to 54 XXX and YYY. Some known slippery sites in -1 PRF show divergence from this pattern, e.g. in the 55 NSA2 gene of west nile virus with the slippery site sequence UCCUUUU (Firth and Atkins, 2009 ; 56 expression levels (by gating for a narrow range of mCherry fluorescence to minimize effects coming 96 from the influence of the variable region on overall expression levels, Methods). This percentage value 97 does not denote the precise rate of frameshifting events, but gives us a meaningful measure of 98 frameshifting efficiencies across PRF events. We thereby obtained measurements for 8972 (67%) 99
Search related documents:
Co phrase search for related documents- expression level and frameshifting efficiency: 1
- expression level and overall expression level: 1, 2, 3, 4, 5, 6, 7, 8
- expression level and percentage value: 1, 2
- frameshifting efficiency and narrow range: 1
Co phrase search for related documents, hyperlinks ordered by date