Author: lianhua dong; Junbo Zhou; Chunyan Niu; Quanyi Wang; Yang Pan; Xia Wang; Yongzhuo zhang; Jiayi yang; Manqing Liu; Yang zhao; Tao peng; Jie xie; yunhua gao; Di wang; yun zhao; Xinhua Dai; xiang fang
Title: Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR Document date: 2020_3_18
ID: aa7slcnc_30
Snippet: Currently, besides RT-qPCR, other methods such as next generation sequencing The overall sensitivity and diagnostic accuracy of RT-qPCR in our study were 36% and 53%, respectively, according to our follow-up survey on the 188 samples(6 out of 194 can not be tracked). The RT-qPCR sensitivity is in agreement with the previous report for the throat swab samples (5). However, both sensitivity and diagnostic accuracy of RT-dPCR dramatically increased .....
Document: Currently, besides RT-qPCR, other methods such as next generation sequencing The overall sensitivity and diagnostic accuracy of RT-qPCR in our study were 36% and 53%, respectively, according to our follow-up survey on the 188 samples(6 out of 194 can not be tracked). The RT-qPCR sensitivity is in agreement with the previous report for the throat swab samples (5). However, both sensitivity and diagnostic accuracy of RT-dPCR dramatically increased to 90 % and 93%, respectively. Furthermore, it is very sensitive for the very low viral load in close contacts and suitable for monitoring the change of the viral load in the convalescent patients. An additional advantage of quantification of SARS-CoV-2 copy number by RT-dPCR is that comparisons can be conducted between different dates and different laboratories as absolute quantitation of targets by RT-dPCR provides high concordance between All rights reserved. No reuse allowed without permission.
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