Identification of Human Immune Cell Subtypes Most Vulnerable to IL-1ß-induced Inflammatory Signaling Using Mass Cytometry

Hema Kothari; Corey M. Williams; Chantel McSkimming; Mythili Vigneshwar; Eli R. Zunder; Coleen A. McNamara

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.19.047274 doi: bioRxiv preprint Fig 5. CD16 -CD56 -CD161 + cells that express CD25 shows remarkable induction of p-NF-kB and p-p38 expression upon IL-1β stimulation. UMAP followed by Leiden clustering was performed on gated CD3 -CD19 -HLA-DRcells using both vehicle and IL-1β-stimulated cells using cell surface markers as shown in c. a, 17 cell clusters were identified as shown superimposed on the UMAP. b, Frequency of clusters and inter-individual variations. c, Heatmap showing median intensity of surface markers across all 17 clusters. Frequency of d, p-NF-kB + cells and e p-p38 + cells within each cluster in the vehicle-treated and IL-1β-stimulated samples. Significant differences represent the comparisons between vehicle and IL-1β-stimulated samples. Data shown is mean ± SEM. Paired t-test. N=3; * p-value ≤ 0.05.