Selected article for: "cell lysis and lysis buffer"
Author: Ting Gao; Mingdong Hu; Xiaopeng Zhang; Hongzhen Li; Lin Zhu; Hainan Liu; Qincai Dong; Zhang Zhang; Zhongyi Wang; Yong Hu; Yangbo Fu; Yanwen Jin; Kaitong Li; Songtao Zhao; Yongjiu Xiao; Shuping Luo; Lufeng Li; Lingfang Zhao; Junli Liu; Huailong Zhao; Yue Liu; Weihong Yang; Jing Peng; Xiaoyu Chen; Ping Li; Yaoning Liu; Yonghong Xie; Jibo Song; Lu Zhang; Qingjun Ma; Xiuwu Bian; Wei Chen; Xuan Liu; Qing Mao; Cheng Cao
Title: Highly pathogenic coronavirus N protein aggravates lung injury by MASP-2-mediated complement over-activation
  • Document date: 2020_3_30
    • ID: dxs8ggyh_31
    Snippet: The Immunoprecipitation and immunoblotting 20 Cell lysates were prepared in lysis buffer (50 mM Tris-HCl, pH 7.5, 1 mM phenylmethylsulfonyl fluoride, 1 mM dithiothreitol, 10 mM sodium fluoride, 10 µg/ml aprotinin, 10 µg/ml leupeptin, and 10 µg/ml pepstatin A) containing 1% Nonidet P-40. Soluble proteins were subjected to immunoprecipitation with anti-Flag M2 agarose (Sigma). The adsorbates were then separated by SDS-PAGE and transferred onto a.....
    Document: The Immunoprecipitation and immunoblotting 20 Cell lysates were prepared in lysis buffer (50 mM Tris-HCl, pH 7.5, 1 mM phenylmethylsulfonyl fluoride, 1 mM dithiothreitol, 10 mM sodium fluoride, 10 µg/ml aprotinin, 10 µg/ml leupeptin, and 10 µg/ml pepstatin A) containing 1% Nonidet P-40. Soluble proteins were subjected to immunoprecipitation with anti-Flag M2 agarose (Sigma). The adsorbates were then separated by SDS-PAGE and transferred onto an Immobilon-P transfer membrane 25 (Millipore) by semi-dry transblot (Biorad). The membrane was blocked by 5% Western-Blocker (Biorad). Immunoblot analysis was performed with horseradish peroxidase (HRP)-conjugated anti-Flag (Sigma), anti-β-actin (Sigma), anti-green fluorescent protein (GFP) (Clontech), anti-MASP-2 (Santa Cruz), anti-C4α (Santa Cruz), HRP-conjugated anti-Myc (Santa Cruz), and goat anti-mouse immunoglobulin G (IgG) (Amersham/Pharmacia) antibodies. The antigen-antibody 30 complexes were visualized by chemiluminescence (GE Health Care).

    Search related documents:
    Co phrase search for related documents
    • antigen antibody and horseradish peroxidase: 1, 2, 3, 4, 5, 6, 7, 8
    • antigen antibody and HRP conjugate: 1, 2, 3
    • antigen antibody and lysis buffer: 1, 2
    • cell lysate and Immunoblot analysis: 1
    • cell lysate and lysis buffer: 1, 2, 3, 4, 5
    • horseradish peroxidase and HRP conjugate: 1, 2
    • horseradish peroxidase and Immunoblot analysis: 1, 2
    • horseradish peroxidase and lysis buffer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
    • Immunoblot analysis and lysis buffer: 1, 2, 3