Identification of peptide coatings that enhance diffusive transport of nanoparticles through the tumor microenvironment

Rashmi Mohanty; Xinquan Liu; Jae You Kim; Xiujuan Peng; Sahil Bhandari; Jasmim Leal; Dhivya Arasappan; Dennis C. Wylie; Tony Dong; Debadyuti Ghosh

Next, we quantified the MSD of the uncoated particle through PBS using particle tracking (Script S1 and Figure S4 ) and used these measurements to understand how the tumor ECM hinders nanoparticle transport ( Table 3) . The ensemble-averaged MSD of the particles in tumor ECM was compared to the unhindered MSD of the uncoated bead through PBS after 1 sec time lag ( Table 3) . There was a ~2000-fold decrease in the MSD of the uncoated nanoparticle through the tumor, whereas peptide and PEG-coatings reduced the hindrance of nanoparticle transport through the tumor tissue. P4 surface-coating most effectively reduced hindered transport, with only a ~57-fold decrease in transport in tumor relative to unhindered transport in PBS.