Selected article for: "generation sequencing and NGS generation sequencing"
Author: Rashmi Mohanty; Xinquan Liu; Jae You Kim; Xiujuan Peng; Sahil Bhandari; Jasmim Leal; Dhivya Arasappan; Dennis C. Wylie; Tony Dong; Debadyuti Ghosh
Title: Identification of peptide coatings that enhance diffusive transport of nanoparticles through the tumor microenvironment
  • Document date: 2019_6_4
    • ID: e2uzk4u1_72
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/659524 doi: bioRxiv preprint From each round of screening from each replicate, a fraction of the phage eluate was prepared for next-generation DNA sequencing (NGS) to identify DNA sequences that encode for the peptide-presenting phage that permeate through the ECM. Prior to NGS preparation, ten microliters of each library sample (~.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/659524 doi: bioRxiv preprint From each round of screening from each replicate, a fraction of the phage eluate was prepared for next-generation DNA sequencing (NGS) to identify DNA sequences that encode for the peptide-presenting phage that permeate through the ECM. Prior to NGS preparation, ten microliters of each library sample (~10 8 pfu/µL) was denatured at 100 °C for 15 minutes to lyse the phage and release the encapsulated DNA. The workflow for NGS sample preparation was based on manufacturers recommendation (Illumina 16S Metagenomic Sequencing Protocol).

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