SCV-2000bp: a primer panel for SARS-CoV-2 full-genome sequencing

Chen, Hongbo; Xie, Zhongping; Long, Runxiang; Fan, Shengtao; Li, Heng; He, Zhanlong; Xu, Kanwei; Liao, Yun; Wang, Lichun; Zhang, Ying; Li, Xueqi; Dong, Xingq; Mou, Tangwei; Zhou, Xiaofang; Yang, Yaoyun; Guo, Lei; Yang, Jianbo; Zheng, Huiwen; Xu, Xingli; Li, Jing; Liang, Yan; Li, Dandan; Zhao, Zhimei; Hong, Chao; Zhao, Heng; Jiang, Guorun; Che, Yanchun; Yang, Fengmei; Hu, Yunguang; Wang, Xi; Pu, Jing; Ma, Kaili; Chen, Chen; Duan, Weiguo; Shen, Dong; Zhao, Hongling; Jiang, Ruiju; Deng, Xinqiang; Li, Yan; Zhu, Hailian; Zhou, Jian; Yu, Li; Xu, Mingjue; Yang, Huijuan; Yi, Li; Zhou, Zhenxin; Yang, Jiafang; Duan, Nan; Yang, Huan; Zhao, Wangli; Yang, Wei; Li, Changgui; Liu, Longding; Li, Qihan

Here we provide technical data for amplifying the complete genome of SARS-CoV-2 from clinical samples using only seventeen pairs of primers. We demonstrate that the [C]V2000bp primer panel successfully produces genomes when used with the residual total RNA extracts from positive clinical samples following diagnostic RT-PCRs (with Ct in the range from 13 to 20). The library preparation method reported here includes genome amplification of ~1750-2000 bp fragments followed by ultrasonic fragmentation combined with the introduction of Illumina compatible adapters. Using the SCV2000bp panel, 25 complete SARS-CoV-2 virus genome sequences were sequenced from clinical samples of COVID-19 patients from Moscow obtained in late March - early April.