Author: Renata C Fleith; Harriet V Mears; Edward Emmott; Stephen C Graham; Daniel S Mansur; Trevor R Sweeney
Title: IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA Document date: 2018_2_8
ID: j97gul0w_13
Snippet: Wild type or mutant IFIT1 was combined with IFIT3 as described in IFIT complex assembly, above. 150 ïL of each assembly reaction was injected on to a Superdex 200 Increase 10/300 GL column, at 0.3 mL/min flow rate and UV280 readings were monitored. Peak fractions were analysed by SDS-PAGE and Coomassie staining......
Document: Wild type or mutant IFIT1 was combined with IFIT3 as described in IFIT complex assembly, above. 150 ïL of each assembly reaction was injected on to a Superdex 200 Increase 10/300 GL column, at 0.3 mL/min flow rate and UV280 readings were monitored. Peak fractions were analysed by SDS-PAGE and Coomassie staining.
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