Author: Wen Wen; Wenru Su; Hao Tang; Wenqing Le; Xiaopeng Zhang; Yingfeng Zheng; XiuXing Liu; Lihui Xie; Jianmin Li; Jinguo Ye; Xiuliang Cui; Yushan Miao; Depeng Wang; Jiantao Dong; Chuan-Le Xiao; Wei Chen; Hongyang Wang
Title: Immune Cell Profiling of COVID-19 Patients in the Recovery Stage by Single-Cell Sequencing Document date: 2020_3_27
ID: 8aezcyf9_76
Snippet: Full-length TCR/BCR V(D)J segments were enriched from amplified cDNA from 5' libraries via PCR amplification using a Chromium Single-Cell V(D)J Enrichment kit according to the manufacturer's protocol (10x Genomics). The TCR/BCR sequences for each single T/B cell were assembled by Cell Ranger vdj pipeline (v3.1.0), leading to the identification of CDR3 sequence and the rearranged TCR/BCR gene. Analysis was performed using Loupe V(D)J Browser v.2.0.....
Document: Full-length TCR/BCR V(D)J segments were enriched from amplified cDNA from 5' libraries via PCR amplification using a Chromium Single-Cell V(D)J Enrichment kit according to the manufacturer's protocol (10x Genomics). The TCR/BCR sequences for each single T/B cell were assembled by Cell Ranger vdj pipeline (v3.1.0), leading to the identification of CDR3 sequence and the rearranged TCR/BCR gene. Analysis was performed using Loupe V(D)J Browser v.2.0.1 (10x Genomics). In brief, a TCR/BCR diversity metric, containing clonotype frequency and barcode information, was obtained. Using barcode information, T/B cells with prevalent TCR/BCR clonotypes were projected on a t-SNE plot.
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