Author: François Ferron; Humberto Julio Debat; Etienne Decroly; Bruno Canard
Title: Identification of a Nidovirales Orf1a N7-guanine cap Methyltransferase signature-sequence as a genetic marker of large genome Tobaniviridae Document date: 2019_5_17
ID: lnf2mj29_11
Snippet: Sequences were analyzed using HHblits and HHpred tools of the BioInformatics tookit ( [28] ). When available, authentic cleavage sites were used to predict protein gene products of the Orf1ab, Orf1a, and Orf1b polyproteins. The boundaries were otherwise approximately (+/-10 aa) determined using structural homologies detected using HHPred, except for the N-term boundary of the Orf1b gene product: In Nidovirales, the absence of any structural data .....
Document: Sequences were analyzed using HHblits and HHpred tools of the BioInformatics tookit ( [28] ). When available, authentic cleavage sites were used to predict protein gene products of the Orf1ab, Orf1a, and Orf1b polyproteins. The boundaries were otherwise approximately (+/-10 aa) determined using structural homologies detected using HHPred, except for the N-term boundary of the Orf1b gene product: In Nidovirales, the absence of any structural data nor homology (outside the order) on the N-terminus of the RdRp gene (nsp9 Arteriviridae, nsp12 in Coronaviridae), which was used for phylogenic analysis, precludes precise sequence homology search in this limited area comprised between the nsp10 and nsp12 proteins (Coronavirus gene-product naming).
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