Author: François Ferron; Humberto Julio Debat; Etienne Decroly; Bruno Canard
Title: Identification of a Nidovirales Orf1a N7-guanine cap Methyltransferase signature-sequence as a genetic marker of large genome Tobaniviridae Document date: 2019_5_17
ID: lnf2mj29_12
Snippet: Multiple sequence alignments (MSA) were generated using Muscle in SeaView [29] . For each sequence of unknown structure, secondary structures were predicted using Predict Protein [30] . The predicted secondary structures were used to validate the alignment with structural references. The MSA was rendered using ESPript 3.0 [31] , together with appropriate structural models as indicated, to assign secondary structures. When possible, structural 3D .....
Document: Multiple sequence alignments (MSA) were generated using Muscle in SeaView [29] . For each sequence of unknown structure, secondary structures were predicted using Predict Protein [30] . The predicted secondary structures were used to validate the alignment with structural references. The MSA was rendered using ESPript 3.0 [31] , together with appropriate structural models as indicated, to assign secondary structures. When possible, structural 3D models were generated using Phyre 2.0 [32] . Conserved patches of amino-acids were generated using WebLogo [33] and mapped in the structural models rendered in Chimera.
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