Author: Longlong Si; Haiqing Bai; Melissa Rodas; Wuji Cao; Crystal Yur Oh; Amanda Jiang; Atiq Nurani; Danni Y Zhu; Girija Goyal; Sarah Gilpin; Rachelle Prantil-Baun; Donald E. Ingber
Title: Human organs-on-chips as tools for repurposing approved drugs as potential influenza and COVID19 therapeutics in viral pandemics Document date: 2020_4_14
ID: mrgw2mnx_46
Snippet: Plaque formation assay. Virus titers were determined using plaque formation assays. Confluent MDCK cell monolayers in 12-well plate were washed with PBS, inoculated with 1 mL of 10-fold serial dilutions of influenza virus samples, and incubated for 1 h at 37℃. After unabsorbed virus was removed, the cell monolayers were overlaid with 1 mL of DMEM (Gibco) supplemented with 1.5% low melting point agarose (Sigma-Aldrich) and 2 µg/mL TPCK-treated .....
Document: Plaque formation assay. Virus titers were determined using plaque formation assays. Confluent MDCK cell monolayers in 12-well plate were washed with PBS, inoculated with 1 mL of 10-fold serial dilutions of influenza virus samples, and incubated for 1 h at 37℃. After unabsorbed virus was removed, the cell monolayers were overlaid with 1 mL of DMEM (Gibco) supplemented with 1.5% low melting point agarose (Sigma-Aldrich) and 2 µg/mL TPCK-treated trypsin (Sigma-Aldrich). After incubation for 2-4 author/funder. All rights reserved. No reuse allowed without permission.
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