Selected article for: "cell surface and NK cell"
Author: Hema Kothari; Corey M. Williams; Chantel McSkimming; Mythili Vigneshwar; Eli R. Zunder; Coleen A. McNamara
Title: Identification of Human Immune Cell Subtypes Most Vulnerable to IL-1ß-induced Inflammatory Signaling Using Mass Cytometry
  • Document date: 2020_4_19
    • ID: 5rhn7gom_23
    Snippet: CD3 -CD19 -HLA-DRcells (Supplementary Fig. 3e) were used for NK cell clustering to identify IL-1β responsive subsets. These CD3 -CD19 -HLA-DRcells included CD56 bright , CD56 dim as well as CD56populations. In addition to the prototypical NK cell markers such as CD16, CD56 and CD161, other cell surface markers including CXCR3, CXCR4, CD38, CD25, CD11b and CD11c, which were expressed on NK cells were used for the clustering. Interestingly, a tota.....
    Document: CD3 -CD19 -HLA-DRcells (Supplementary Fig. 3e) were used for NK cell clustering to identify IL-1β responsive subsets. These CD3 -CD19 -HLA-DRcells included CD56 bright , CD56 dim as well as CD56populations. In addition to the prototypical NK cell markers such as CD16, CD56 and CD161, other cell surface markers including CXCR3, CXCR4, CD38, CD25, CD11b and CD11c, which were expressed on NK cells were used for the clustering. Interestingly, a total of 17 distinct clusters were identified (Fig. 5a-c) . Analysis of IL-1β-induced signaling demonstrated a 10-15-fold induction in the frequency of p-NF-kB + cells in the CD16 -CD56 dim CD161 + NK cells (cluster 2) and CD16 -CD56 bright CD161 -(cluster 3) (Fig. 5d) . Notably, another CD161 + cluster that expressed CD25 but was negative for both CD16 and CD56 (cluster 17) showed the most striking expression of p-NF-kB (>70-fold vs vehicle control) (Fig. 5d) . Moreover, IL-1β-induced expression of p-p38 expression was observed only in this cluster (Fig. author/funder. All rights reserved. No reuse allowed without permission.

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