Selected article for: "RNA cap and signature sequence"
Author: François Ferron; Humberto Julio Debat; Etienne Decroly; Bruno Canard
Title: Identification of a Nidovirales Orf1a N7-guanine cap Methyltransferase signature-sequence as a genetic marker of large genome Tobaniviridae
  • Document date: 2019_5_17
    • ID: lnf2mj29_22
    Snippet: We thus performed a larger search of MTase signature-sequences along the whole Orf1ab in all Nidovirales: surprisingly, a RF-MTase signature-sequence was detected in Orf1a of 10 members of the Tobaniviridae family (Fig. 2) . Strictly conserved amino-acids in these new viral MTases define three motifs: 3 glycines of the SAM binding site (G54, 56, and 58 in WBV) 2 residues downstream of a 3 amino acid hydrophobic patches in a β-strand structure, a.....
    Document: We thus performed a larger search of MTase signature-sequences along the whole Orf1ab in all Nidovirales: surprisingly, a RF-MTase signature-sequence was detected in Orf1a of 10 members of the Tobaniviridae family (Fig. 2) . Strictly conserved amino-acids in these new viral MTases define three motifs: 3 glycines of the SAM binding site (G54, 56, and 58 in WBV) 2 residues downstream of a 3 amino acid hydrophobic patches in a β-strand structure, a histidine (H117 immediately followed by either F or Y, and Glu175 (Fig. S4) . Interestingly the catalytic K-D-K-E tetrad associated to 2'O-MTase is lacking, suggesting that they could correspond to the missing RNA-cap N7-guanine MTase.

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