Immuno-informatics Characterization SARS-CoV-2 Spike Glycoprotein for Prioritization of Epitope based Multivalent Peptide Vaccine

Saba Ismail; Sajjad Ahmad; Syed Sikander Azam

Salt bridges are non-covalent in nature and the outcome of interactions between two ionized states [101] . These interactions comprised two parts: an electrostatic interaction and a hydrogen bond. In salt bridges, lysine or arginine typically behave as base where glutamine or aspartate as acid and the bridge is created when carboxylic acid group allows a proton migration to guanidine and amine group in arginine. Salt bridges are the strongest among all non-covalent interactions and contribute to a major extent in biomolecular stability [102] [103] [104] . In total, 17 salt bridges were identified between TLR3 and MEPVC within the cut-off distance of 3.2 Ã… as can be depicted from Fig.11C . The higher numbers of salt bridges were recorded for TLR3-Glu628 and MEPVC-Lys685. The mean number of salt bridges for this interaction is 18, max, 35 and min, 3. The count for other salt bridges from TLR3 to MEPVC is in following order: Asp124-Arg706 (mean, 3, max,7 and min,3), Glu98-Lys679 (mean, 5, max,10 and min,4), Asp402-Arg641 (mean, 12, max, 18 and min, 4), Glu146-Arg706 (mean,7 max,11 and min,3), Glu146-Lys679 (mean,5 max,13 and min,2), Glu272-Lys655 author/funder. All rights reserved. No reuse allowed without permission.