Author: Renata C Fleith; Harriet V Mears; Edward Emmott; Stephen C Graham; Daniel S Mansur; Trevor R Sweeney
Title: IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA Document date: 2018_2_8
ID: j97gul0w_10
Snippet: Proteins were resolved by 12.5 % SDS-PAGE and transferred to 0.45 μm nitrocellulose membrane. For immunoprecipitations, membranes were probed with anti-FLAG M2-Peroxidase (A8592, Sigma), detected by chemiluminescence using Westar Supernova substrate (Cyanagen) and visualised on Super RX-N film (Fugifilm). As a loading control, membranes were probed with anti-GAPDH (AM4300, ThermoFisher), detected using IRDye 800CW Goat anti-Mouse and visualised .....
Document: Proteins were resolved by 12.5 % SDS-PAGE and transferred to 0.45 μm nitrocellulose membrane. For immunoprecipitations, membranes were probed with anti-FLAG M2-Peroxidase (A8592, Sigma), detected by chemiluminescence using Westar Supernova substrate (Cyanagen) and visualised on Super RX-N film (Fugifilm). As a loading control, membranes were probed with anti-GAPDH (AM4300, ThermoFisher), detected using IRDye 800CW Goat anti-Mouse and visualised on an Odyssey CLx Imaging System (Li-Cor). To normalise recombinant IFIT proteins and complexes, membranes were probed with anti-penta-His (34660, Qiagen) and quantified using ImageJ.
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