Author: Renata C Fleith; Harriet V Mears; Edward Emmott; Stephen C Graham; Daniel S Mansur; Trevor R Sweeney
Title: IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA Document date: 2018_2_8
ID: j97gul0w_24
Snippet: Following immunoprecipitation, the combined samples were subjected to SDS-PAGE electrophoresis on a precast gel, and extracted as a single band for in-gel trypsinisation. The resulting peptides were fractionated using an Ultimate 3000 nanoHPLC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). In brief, peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scienti.....
Document: Following immunoprecipitation, the combined samples were subjected to SDS-PAGE electrophoresis on a precast gel, and extracted as a single band for in-gel trypsinisation. The resulting peptides were fractionated using an Ultimate 3000 nanoHPLC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). In brief, peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5 % (vol/vol) acetonitrile 0.1 % (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient, using 7 gradient segments (1-6 % solvent B over 1 min., 6-15 % B over 58 min., 15-32 %B over 58 author/funder. All rights reserved. No reuse allowed without permission.
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