Author: Daniel J Butler; Christopher Mozsary; Cem Meydan; David C Danko; Jonathan Foox; Joel Rosiene; Alon Shaiber; Ebrahim Afshinnekoo; Matthew MacKay; Fritz J Sedlazeck; Nikolay A Ivanov; Maria A Sierra; Diana Pohle; Michael Zeitz; Vijendra Ramlall; Undina Gisladottir; Craig D Westover; Krista Ryon; Benjamin Young; Chandrima Bhattacharya; Phyllis Ruggiero; Bradley W Langhorst; Nathan A Tanner; Justyn Gawrys; Dmitry Meleshko; Dong Xu; Jenny Xiang; Angelika Iftner; Daniela Bezdan; John Sipley; Lin Cong; Arryn Craney; Priya Velu; Ari Melnick; Iman A Hajirasouliha; Thomas Iftner; Mirella Salvatore; Massimo Loda; Lars F Westblade; Shawn Levy; Melissa Cushing; Nicholas P Tatonetti; Marcin Imielinski; Hanna Rennert; Christopher Mason
Title: Host, Viral, and Environmental Transcriptome Profiles of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Document date: 2020_4_20
ID: kyoa5gsf_32
Snippet: These data demonstrate unique host transcriptome profiles during viral infection with SARS-CoV-2, as well as clade-specific mutational landscapes of the virus, both representing the early phase of the U.S. pandemic (March 2020). Total RNA-seq (ribo-depleted RNA) used here enabled a comprehensive molecular map for the virus and host, including complete genotypes (including insertions and deletions) across the length of the viral genome and coding .....
Document: These data demonstrate unique host transcriptome profiles during viral infection with SARS-CoV-2, as well as clade-specific mutational landscapes of the virus, both representing the early phase of the U.S. pandemic (March 2020). Total RNA-seq (ribo-depleted RNA) used here enabled a comprehensive molecular map for the virus and host, including complete genotypes (including insertions and deletions) across the length of the viral genome and coding and noncoding RNA gene expression quantification. Moreover, we show that LAMP is a quick (30minute) and effective assay that could be readily deployed to aid current and future viral testing and surveillance, since it is comparable to RNA-seq and qRT-PCR and can be used with author/funder. All rights reserved. No reuse allowed without permission.
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