Identification of Human Immune Cell Subtypes Most Vulnerable to IL-1ß-induced Inflammatory Signaling Using Mass Cytometry

Hema Kothari; Corey M. Williams; Chantel McSkimming; Mythili Vigneshwar; Eli R. Zunder; Coleen A. McNamara

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.19.047274 doi: bioRxiv preprint Fig 2. Effector-memory T cell subsets are the predominant subsets that express IL-1β induced p-NF-kB and p-p38. UMAP followed by Leiden clustering was performed on CD3 + T cells using both vehicle and IL-1β-stimulated cells and cell surface markers as shown in c. a, 12 T cell clusters were identified as shown superimposed on the UMAP. b, Frequency of T cell clusters and inter-individual variations between donors. c, Heatmap showing median expression of surface markers across all 12 T cell clusters. The color in the heatmap represents the median of the arcsinh for each subset with 0-1 transformed marker expression. The dendrogram represents the hierarchical similarity for subsets. Frequency of d, p-NF-kB + cells and e, p-p38 + cells within each cluster in the vehicle-treated and IL-1β-stimulated samples. Significant differences represent the comparisons between vehicle and IL-1βstimulated samples. Data shown is mean ± SEM. Paired t-test. N=3; * p-value ≤ 0.05. author/funder. All rights reserved. No reuse allowed without permission.