Imaging Striatal Dopamine Release Using a Non-Genetically Encoded Near-Infrared Fluorescent Catecholamine Nanosensor

Abraham G. Beyene; Kristen Delevich; Jackson Travis Del Bonis-O’Donnell; David J. Piekarski; Wan Chen Lin; A. Wren Thomas; Sarah J. Yang; Polina Kosillo; Darwin Yang; Linda Wilbrecht; Markita P. Landry

(GT)6 oligonucleotides were purchased from Integrated DNA Technologies (IDT, Standard Desalting). HiPCo SWNT were purchased from NanoIntegris (Batch # HR27-104). (GT)6-SWNT colloidal suspension (nIRCat) was prepared by mixing 1 mg of (GT)6 and 1 mg of SWNT in 1 mL of a 100 mM NaCl solution. The solution was bath sonicated (Branson Ultrasonic 1800) for 10 minutes and probe-tip sonicated for 10 minutes at 5 W power (Cole Parmer Ultrasonic Processor, 3 mm tip diameter) in an ice-bath. The sonicated solution was incubated at room temperature for 30 minutes. The product was subsequently centrifuged at 16,000 g (Eppendorf 5418) for 90 minutes to remove unsuspended SWNT bundles and amorphous carbon, and the supernatant was recovered for characterization and use. Each nanosensor suspension was stored at 4°C until use.