Selected article for: "mutant IFIT1 wild type and wild type"
Author: Renata C Fleith; Harriet V Mears; Edward Emmott; Stephen C Graham; Daniel S Mansur; Trevor R Sweeney
Title: IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA
  • Document date: 2018_2_8
    • ID: j97gul0w_51
    Snippet: Disruption of dimerisation was previously reported not to affect the translation inhibition activity of IFIT1 (BioRxiv: https://doi.org/10.1101/152850 and Figure 5G , white bars). Therefore, we examined what impact mutations in the 'YxxxL' motif had on the ability of IFIT3 to stimulate IFIT1 translation inhibition activity. IFIT1 mutants were combined with IFIT3, reporter mRNA and RRL as described in Materials and Methods and Fluc dependent lumin.....
    Document: Disruption of dimerisation was previously reported not to affect the translation inhibition activity of IFIT1 (BioRxiv: https://doi.org/10.1101/152850 and Figure 5G , white bars). Therefore, we examined what impact mutations in the 'YxxxL' motif had on the ability of IFIT3 to stimulate IFIT1 translation inhibition activity. IFIT1 mutants were combined with IFIT3, reporter mRNA and RRL as described in Materials and Methods and Fluc dependent luminescence was measured ( Figure 5G ). At 40 nM IFIT1, 'YxxxL' motif mutants displayed similar translation inhibition. IFIT3 significantly enhanced translation inhibition of both wild type IFIT1 and the IFIT1-L464E mutant. In contrast, IFIT3 did not stimulate translation inhibition of either the IFIT1-Y460E mutant or the L464E/Y460E double mutant.

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