Author: Abraham G. Beyene; Kristen Delevich; Jackson Travis Del Bonis-O’Donnell; David J. Piekarski; Wan Chen Lin; A. Wren Thomas; Sarah J. Yang; Polina Kosillo; Darwin Yang; Linda Wilbrecht; Markita P. Landry
Title: Imaging Striatal Dopamine Release Using a Non-Genetically Encoded Near-Infrared Fluorescent Catecholamine Nanosensor Document date: 2018_7_3
ID: n75siuwb_42
Snippet: Adult male and female mice (>P60) were used for all surgeries. Bilateral viral injections were performed using previously described procedures 47 at the following stereotaxic coordinates: dorsomedial prefrontal cortex (dmPFC): 1.94 mm from Bregma, 0.34 mm lateral from midline, and 0.70 mm vertical from cortical surface; substantia nigra pars compacta (SNc): -3.08 mm from Bregma, 1.25 mm lateral from midline, and 4.0 mm vertical from cortical surf.....
Document: Adult male and female mice (>P60) were used for all surgeries. Bilateral viral injections were performed using previously described procedures 47 at the following stereotaxic coordinates: dorsomedial prefrontal cortex (dmPFC): 1.94 mm from Bregma, 0.34 mm lateral from midline, and 0.70 mm vertical from cortical surface; substantia nigra pars compacta (SNc): -3.08 mm from Bregma, 1.25 mm lateral from midline, and 4.0 mm vertical from cortical surface. For glutamatergic corticostriatal axon stimulation experiments, mice were injected with 0.5 µL of CAG-ChR2-EYFP virus bilaterally into dmPFC. For nigrostriatal dopaminergic axon stimulation experiments, DAT-Cre mice were injected with 0.5 µL DIO-ChR2-EYFP virus bilaterally. For all optogenetic experiments, we waited at least three weeks from viral injection to experimental stimulation to allow for sufficient ChR2 gene expression.
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