Selected article for: "cytometry analysis flow and flow cytometry"
Author: Jun Wu; Dingyun Song; Zhongwen Li; Baojie Guo; Yani Xiao; Wenjing Liu; Lingmin Liang; Chunjing Feng; Tingting Gao; Zai Wang; Jianyan Wen; Shengnan Yang; Peipei Liu; Lei Wang; Yukai Wang; Liang Peng; Guihai Feng; Shiqiang Huang; Glyn Nigel Stacey; Wei Li; Yan Huo; Ronghua Jin; Qi Zhou; Liu Wang; Baoyang Hu; Huaping Dai; Jie Hao
Title: Immunity-and-Matrix-Regulatory Cells Derived from Human Embryonic Stem Cells Safely and Effectively Treat Mouse Lung Injury and Fibrosis
  • Document date: 2020_4_16
    • ID: 8dpla6jt_38
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.15.042119 doi: bioRxiv preprint 15 primary umbilical cord-derived MSCs (UCMSCs). All pluripotency genes (POU5F1, SOX2, NANOG, 371 ZFP42, SALL4), mesendoderm genes (MIXL1), and ectoderm genes (GAD1, GFAP) were extinguished 372 in both IMRCs and UCMSCs (Fig. 1E ). IMRCs expressed MSC-specific genes, including PDGFRA and 373 SPARC, and the M.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.15.042119 doi: bioRxiv preprint 15 primary umbilical cord-derived MSCs (UCMSCs). All pluripotency genes (POU5F1, SOX2, NANOG, 371 ZFP42, SALL4), mesendoderm genes (MIXL1), and ectoderm genes (GAD1, GFAP) were extinguished 372 in both IMRCs and UCMSCs (Fig. 1E ). IMRCs expressed MSC-specific genes, including PDGFRA and 373 SPARC, and the MSC-specific surface markers NT5E (CD73), ENG (CD90), THY1 (CD105) and ITGB1 374 (CD29). Flow cytometry analysis further confirmed this surface marker profile (Fig. 1F, Fig.S1A, B) . By 375 contrast, IMRCs were negative for the hematopoietic surface markers PTPRC (CD45) and CD34. IMRCs 376 displayed the ability to undergo tri-lineage differentiation into mesenchymal tissues, such as adipose, 377 cartilage and bone cells (Fig. 1G, Fig.S1C ). The proliferation rate of IMRCs was higher than that of 378

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