Author: Hasan, M. R.; Kalikiri, M. K. R.; Mirza, F.; Sundararaju, S.; Sharma, A.; Lorenz, S.; Chemaitelly, H.; El-Kahlout, R. A.; Tsui, K. M.; Yassine, H. M.; Coyle, P. V.; Al Khal, A.; Bertollini, R.; Al Thani, M. H.; Abu-Raddad, L. J.; Tang, P.
Title: Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar Cord-id: cz8zy09o Document date: 2021_7_22
ID: cz8zy09o
Snippet: Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792 nasopharyngeal PCR-positive samples collected between April-June 2021 were successfully
Document: Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792 nasopharyngeal PCR-positive samples collected between April-June 2021 were successfully genotyped, revealing the importation and transmission dynamics of these three variants in Qatar.
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