Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants (PPR) virus in cell culture

Siddiqui, Md. Saiful Islam; Islam, Md. Rafiqul; Chowdhury, Emdadul Haque

Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants virus was studied in Vero cells to determine maximum virus yield. One-step growth curve was formulated after determining virus in both supernatant (CFV) and cell lysate (CAV) at different time categories by microtitre plate titration in Vero cells and the viral presence was confirmed by real-time RT-PCR. The virus was first detected in both the supernatants and cell pellets at 12 hpi. The virus titre reached its plateau at 72 hpi. Maximum virus titre of CAV was 6.2 log(10) TCID(50)/ml and that of CFV was 5.2 log(10) TCID(50)/ml at 72 hpi. After that, the titer gradually declined, but maintained at 4.5 log(10) TCID(50)/ml in case of CAV and 4.2 log(10) TCID(50)/ml in case of CFV at 96 hpi. It was concluded that the optimum time point for harvesting Vero cell culture is 72 hpi.