Author: Freire-Paspuel, Byron; Vega, Patricio; Velez, Alberto; Castello, Paulina; Cruz, Marilyn; Bereguiain, Miguel Angel Garcia
Title: Evaluation of nCoV-QS (MiCo BioMed) for RT-qPCR detection of SARS-CoV-2 from nasopharyngeal samples using CDC FDA EUA qPCR kit as a gold standard: an example of the need of validation studies Cord-id: cqm4ntvs Document date: 2020_5_6
ID: cqm4ntvs
Snippet: Several qPCR kits are available for SARS-CoV-2 diagnosis, mostly lacking of evaluation due to covid19 emergency. We evaluated nCoV-QS (MiCo BioMed) kit using CDC kit as gold standard. We found limitations for nCoV-QS: 1) lower sensibility 2) lack of RNA quality control probe 3) no capacity to quantify viral load.
Document: Several qPCR kits are available for SARS-CoV-2 diagnosis, mostly lacking of evaluation due to covid19 emergency. We evaluated nCoV-QS (MiCo BioMed) kit using CDC kit as gold standard. We found limitations for nCoV-QS: 1) lower sensibility 2) lack of RNA quality control probe 3) no capacity to quantify viral load.
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