Selected article for: "amplification reaction and diagnostic tool"
Author: Arizti-Sanz, Jon; Freije, Catherine A.; Stanton, Alexandra C.; Boehm, Chloe K.; Petros, Brittany A.; Siddiqui, Sameed; Shaw, Bennett M.; Adams, Gordon; Kosoko-Thoroddsen, Tinna-Solveig F.; Kemball, Molly E.; Gross, Robin; Wronka, Loni; Caviness, Katie; Hensley, Lisa E.; Bergman, Nicholas H.; MacInnis, Bronwyn L.; Lemieux, Jacob E.; Sabeti, Pardis C.; Myhrvold, Cameron
Title: Integrated sample inactivation, amplification, and Cas13-based detection of SARS-CoV-2
  • Cord-id: 928r9t4p
  • Document date: 2020_5_28
    • ID: 928r9t4p
    Snippet: The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (SHERLOCK and HUDSON Integration to Navigate Epidemics), a sensitive and specific integrated diagnostic tool that can detect SARS-CoV-2 RNA from unextracted samples. We combine the steps of SHERLOCK into a single-step reaction and optimize HUDSON to accelerate viral inactivation in nasopharyngeal swabs and saliva. SHINE’s results can be visualized wi
    Document: The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (SHERLOCK and HUDSON Integration to Navigate Epidemics), a sensitive and specific integrated diagnostic tool that can detect SARS-CoV-2 RNA from unextracted samples. We combine the steps of SHERLOCK into a single-step reaction and optimize HUDSON to accelerate viral inactivation in nasopharyngeal swabs and saliva. SHINE’s results can be visualized with an in-tube fluorescent readout — reducing contamination risk as amplification reaction tubes remain sealed — and interpreted by a companion smartphone application. We validate SHINE on 50 nasopharyngeal patient samples, demonstrating 90% sensitivity and 100% specificity compared to RT-PCR with a sample-to-answer time of 50 minutes. SHINE has the potential to be used outside of hospitals and clinical laboratories, greatly enhancing diagnostic capabilities.

    Search related documents:
    Co phrase search for related documents
    • absence presence and lod detection: 1
    • absence presence and machine learning: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
    • absence presence and machine learning model: 1, 2, 3, 4
    • acid diagnostic and lod detection: 1, 2, 3, 4
    • acid diagnostic and machine learning: 1, 2
    • acid diagnostic testing and lod detection: 1
    • acid extraction and lod detection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
    • acid extraction and machine learning: 1
    • acid extraction and machine learning model: 1
    • acid testing and lod detection: 1, 2, 3, 4, 5, 6, 7
    • acid testing and machine learning: 1, 2, 3, 4, 5
    • acid testing and machine learning model: 1, 2