Author: Martin Bartas; Václav Brázda; Natália Bohálová; Alessio Cantara; Adriana Volná; Tereza Stachurová; Katerina Malachová; Eva B. Jagelská; Otília Porubiaková; Jirí Cerven; Petr Pecinka
Title: In-depth Bioinformatic Analyses of Human SARS-CoV-2, SARS-CoV, MERS-CoV, and Other Nidovirales Suggest Important Roles of Noncanonical Nucleic Acid Structures in Their Lifecycles Document date: 2020_4_11
ID: d3q0xel1_16
Snippet: In order to be able to display higher structures of the coronavirus genome, we used Galaxy's freeonline webserver (Afgan et al., 2018) and its RNA fold tool (Lorenz et al., 2011) . This tool allows quick calculation of minimum free energy of secondary structures. We left the default parameters (Temperature 37 °C, Unpaired bases to participate in all dangling ends, Naview layout). We used SARS-CoV-2 genomic RNA sequence (NC_045512.2) in FASTA for.....
Document: In order to be able to display higher structures of the coronavirus genome, we used Galaxy's freeonline webserver (Afgan et al., 2018) and its RNA fold tool (Lorenz et al., 2011) . This tool allows quick calculation of minimum free energy of secondary structures. We left the default parameters (Temperature 37 °C, Unpaired bases to participate in all dangling ends, Naview layout). We used SARS-CoV-2 genomic RNA sequence (NC_045512.2) in FASTA format as the input format. The output data were then displayed using the RNA plot tool [3] . We again left the default parameters (Naview layout, Output format Postscript .ps). We then downloaded the displayed secondary structures in high-resolution format. The raw data are provided in Supplementary Material 5.
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