Author: Jennifer N Rauch; Eric Valois; Sabrina C Solley; Friederike Braig; Ryan S Lach; Naomi J Baxter; Kenneth S Kosik; Carolina Arias; Diego Acosta-Alvear; Maxwell Z Wilson
Title: A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material Document date: 2020_4_21
ID: hy1xrw6q_4
Snippet: Cas12 and Cas13 (CRISPR-based) methods have been transformative with regards to pathogen detection. They are sensitive and, when coupled to isothermal amplification methods and lateral flow immunochromatography detection, have been made field deployable. These methods are promising tools for the detection of SARS-CoV-2 [15] [16] [17] [18] . Yet, because of the global demand for testing, key reagents in these protocols are difficult to obtain. To .....
Document: Cas12 and Cas13 (CRISPR-based) methods have been transformative with regards to pathogen detection. They are sensitive and, when coupled to isothermal amplification methods and lateral flow immunochromatography detection, have been made field deployable. These methods are promising tools for the detection of SARS-CoV-2 [15] [16] [17] [18] . Yet, because of the global demand for testing, key reagents in these protocols are difficult to obtain. To lower the barrier to COVID-19 diagnostics, we devised a method we call CREST (Cas13-based, Rugged, Equitable, Scalable Testing). CREST addresses three of the main hurdles-reagent accessibility, equipment availability, and cost-that limit the scalability of Cas13-based testing, by taking advantage of widely available enzymes, low-cost thermocyclers, and easy-to-use fluorescent visualizers. Moreover, CREST is equivalent in sensitivity to the gold standard reverse transcription quantitative polymerase chain reaction (RT-qPCR) method most often deployed for COVID-19 testing. With these advantages, CREST has the potential to facilitate early detection of positive cases, regular monitoring of individuals at high risk, and implementation of informed containment measures for infected individuals.
Search related documents:
Co phrase search for related documents- amplification method and early detection: 1, 2, 3, 4
- amplification method and equipment availability: 1
- amplification method and field deployable: 1, 2
- amplification method and gold standard sensitivity: 1
- amplification method and high risk: 1, 2, 3, 4, 5, 6
- amplification method and infected individual: 1
- amplification method and isothermal amplification method: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- amplification method and lateral flow: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- amplification method and low cost: 1, 2, 3, 4, 5, 6, 7, 8
- amplification method and pathogen detection: 1, 2, 3, 4, 5, 6, 7, 8, 9
- amplification method and positive case: 1
- amplification method and quantitative polymerase: 1, 2, 3, 4, 5, 6, 7, 8
- amplification method and transcription quantitative polymerase: 1, 2, 3, 4, 5, 6
- amplification method and transcription quantitative polymerase chain reaction: 1, 2, 3, 4, 5
- barrier lower and high risk: 1
- chain reaction and early detection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- chain reaction and equipment availability: 1, 2, 3, 4, 5, 6, 7, 8
- chain reaction and Equitable Rugged: 1
- chain reaction and field deployable: 1, 2, 3, 4, 5, 6
Co phrase search for related documents, hyperlinks ordered by date