Author: Qingxin Zhang; Qingshun Zhao
Title: Inactivating porcine coronavirus before nuclei acid isolation with the temperature higher than 56 °C damages its genome integrity seriously Document date: 2020_2_22
ID: 0vjs2w3l_20
Snippet: Compared with the control group that the sample was stored at 4 o C, both DNA and RNA in the samples incubated at 56 o C for 30 -60 minutes also displayed obvious degradation, among which the 28 S and 18 S bands of total RNA of human cells were obviously smeared, and the large bands of genomic DNA became weaker while there were almost no visible genomic DNA and 28 S RNA bands in the sample incubated at 92 o C for 5 minutes (Fig 1D) . However, the.....
Document: Compared with the control group that the sample was stored at 4 o C, both DNA and RNA in the samples incubated at 56 o C for 30 -60 minutes also displayed obvious degradation, among which the 28 S and 18 S bands of total RNA of human cells were obviously smeared, and the large bands of genomic DNA became weaker while there were almost no visible genomic DNA and 28 S RNA bands in the sample incubated at 92 o C for 5 minutes (Fig 1D) . However, the degradation of nucleic acid during the inactivation progress was not as serious as that of the samples prepared by Hank's solution. The results suggest that R503 should have a significant protective effect of both total RNA and genomic DNA from degradation.
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