Author: Qingxin Zhang; Qingshun Zhao
Title: Inactivating porcine coronavirus before nuclei acid isolation with the temperature higher than 56 °C damages its genome integrity seriously Document date: 2020_2_22
ID: 0vjs2w3l_30
Snippet: Furthermore, we examined the amount of phage DNA in the sample prepared with R503 after high temperature treatment by qPCR. The results (Fig. S1D, Table S1) showed that the mean value of CT (21.71 ± 0.04) in the sample prepared by R503 and incubated at 56 o C for 30 minutes had no significant change compared with that in the control group (21.61 ± 0.06) (the difference between the two was less than 0.5). The mean value of CT (22.90 ± 0.26) was.....
Document: Furthermore, we examined the amount of phage DNA in the sample prepared with R503 after high temperature treatment by qPCR. The results (Fig. S1D, Table S1) showed that the mean value of CT (21.71 ± 0.04) in the sample prepared by R503 and incubated at 56 o C for 30 minutes had no significant change compared with that in the control group (21.61 ± 0.06) (the difference between the two was less than 0.5). The mean value of CT (22.90 ± 0.26) was increased by 1.2880 in the sample that was inactivated at 92 o C for 5 minutes, suggesting that 92 o C treatment significantly reduced the detectable amount of DNA viruses in sample even prepared by R503.
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