Inactivating porcine coronavirus before nuclei acid isolation with the temperature higher than 56 °C damages its genome integrity seriously

Qingxin Zhang; Qingshun Zhao

Compared with the CT (31.64 ± 0.10) of the control group that the sample was stored at 4 o C, the mean value of CT (31.31 ± 0.17) of the group incubated at 56 o C for 30 minutes had no significant change. The difference of CTs between the two groups was less than 0.5 ( Fig. 1E ; Figure S1B ; Table S1 ). Moreover, the mean value of CT (31.43 ± 0.08) in the group incubated at 92 o C for 5 minutes was also not changed significantly. The difference of CTs between the two groups was less than 0.5 either (Fig. 1F; Fig. S1B ; Table S1 ). These results showed that the inactivation progress with the temperature higher than 56 o C did not significantly affect the detectable template of viral nucleic acid when it was prepared with R503, suggesting that the fragmented viral RNA could still be good for using as a template for qRT-PCR even if the viral nucleic acid was degraded.