Author: Park, Wan Beom; Kwon, Nak-Jung; Choi, Su-Jin; Kang, Chang Kyung; Choe, Pyoeng Gyun; Kim, Jin Yong; Yun, Jiyoung; Lee, Gir-Won; Seong, Moon-Woo; Kim, Nam Joong; Seo, Jeong-Sun; Oh, Myoung-don
Title: Virus Isolation from the First Patient with SARS-CoV-2 in Korea Cord-id: d1mxoche Document date: 2020_2_18
ID: d1mxoche
Snippet: Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coro
Document: Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.
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